Analysis Note

ControlHuman lung tissue lysate

Application

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Surfactant Protein A in human lung tissue.

Detect SP-A using this rabbit polyclonal antibody, Anti-Surfactant Protein A Antibody validated for use in western blotting & IHC (Paraffin).

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pulmonary surfactant-associated protein A1 (SP-A1, SFTPA1, PSP-A, SP-A) binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38 kDa. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. Genetic variations in SP-A are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) and respiratory distress syndrome in premature infants (RDS).

Immunogen

Epitope: C-type lectin domain

KLH-conjugated linear peptide corresponding to the C-type lectin domain of human Surfactant Protein A.

Linkage

Replaces: AB3420

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Surfactant Protein A in 10 µg of human lung tissue lysate.

Specificity

Other homologies: Uniprot shows no homology to Rat.

This antibody recognizes the C-type lectin domain of Surfactant Protein A.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~35 kDa observed